Mandipropamid targets the cellulose synthase-like PiCesA3 to inhibit cell wall biosynthesis in the oomycete plant pathogen, Phytophthora infestans.
Identifieur interne : 001885 ( Main/Exploration ); précédent : 001884; suivant : 001886Mandipropamid targets the cellulose synthase-like PiCesA3 to inhibit cell wall biosynthesis in the oomycete plant pathogen, Phytophthora infestans.
Auteurs : Mathias Blum [Suisse] ; Martine Boehler ; Eva Randall ; Vanessa Young ; Michael Csukai ; Sabrina Kraus ; Florence Moulin ; Gabriel Scalliet ; Anna O. Avrova ; Stephen C. Whisson ; Raymonde Fonne-PfisterSource :
- Molecular plant pathology [ 1364-3703 ] ; 2010.
Descripteurs français
- KwdFr :
- Acides carboxyliques (pharmacologie), Amides (pharmacologie), Cellulose (biosynthèse), Croisements génétiques (MeSH), Données de séquences moléculaires (MeSH), Dosage génique (génétique), Glucose (métabolisme), Glucosyltransferases (composition chimique), Glucosyltransferases (génétique), Glucosyltransferases (métabolisme), Mutagenèse (effets des médicaments et des substances chimiques), Mutation (génétique), Méthanesulfonate d'éthyle (MeSH), Paroi cellulaire (effets des médicaments et des substances chimiques), Paroi cellulaire (métabolisme), Phytophthora infestans (cytologie), Phytophthora infestans (effets des médicaments et des substances chimiques), Phytophthora infestans (enzymologie), Phytophthora infestans (génétique), Plantes (effets des médicaments et des substances chimiques), Plantes (microbiologie), Protéines d'algue (composition chimique), Protéines d'algue (génétique), Protéines d'algue (métabolisme), Séquence d'acides aminés (MeSH), Transformation génétique (effets des médicaments et des substances chimiques).
- MESH :
- biosynthèse : Cellulose.
- composition chimique : Glucosyltransferases, Protéines d'algue.
- cytologie : Phytophthora infestans.
- effets des médicaments et des substances chimiques : Mutagenèse, Paroi cellulaire, Phytophthora infestans, Plantes, Transformation génétique.
- enzymologie : Phytophthora infestans.
- génétique : Dosage génique, Glucosyltransferases, Mutation, Phytophthora infestans, Protéines d'algue.
- microbiologie : Plantes.
- métabolisme : Glucose, Glucosyltransferases, Paroi cellulaire, Protéines d'algue.
- pharmacologie : Acides carboxyliques, Amides.
- Croisements génétiques, Données de séquences moléculaires, Méthanesulfonate d'éthyle, Séquence d'acides aminés.
English descriptors
- KwdEn :
- Algal Proteins (chemistry), Algal Proteins (genetics), Algal Proteins (metabolism), Amides (pharmacology), Amino Acid Sequence (MeSH), Carboxylic Acids (pharmacology), Cell Wall (drug effects), Cell Wall (metabolism), Cellulose (biosynthesis), Crosses, Genetic (MeSH), Ethyl Methanesulfonate (MeSH), Gene Dosage (genetics), Glucose (metabolism), Glucosyltransferases (chemistry), Glucosyltransferases (genetics), Glucosyltransferases (metabolism), Molecular Sequence Data (MeSH), Mutagenesis (drug effects), Mutation (genetics), Phytophthora infestans (cytology), Phytophthora infestans (drug effects), Phytophthora infestans (enzymology), Phytophthora infestans (genetics), Plants (drug effects), Plants (microbiology), Transformation, Genetic (drug effects).
- MESH :
- chemical , biosynthesis : Cellulose.
- chemical , chemistry : Algal Proteins, Glucosyltransferases.
- chemical , genetics : Algal Proteins, Glucosyltransferases.
- chemical , metabolism : Algal Proteins, Glucose, Glucosyltransferases.
- chemical , pharmacology : Amides, Carboxylic Acids.
- cytology : Phytophthora infestans.
- drug effects : Cell Wall, Mutagenesis, Phytophthora infestans, Plants, Transformation, Genetic.
- enzymology : Phytophthora infestans.
- genetics : Gene Dosage, Mutation, Phytophthora infestans.
- metabolism : Cell Wall.
- microbiology : Plants.
- Amino Acid Sequence, Crosses, Genetic, Ethyl Methanesulfonate, Molecular Sequence Data.
Abstract
Oomycete plant pathogens cause a wide variety of economically and environmentally important plant diseases. Mandipropamid (MPD) is a carboxylic acid amide (CAA) effective against downy mildews, such as Plasmopara viticola on grapes and potato late blight caused by Phytophthora infestans. Historically, the identification of the mode of action of oomycete-specific control agents has been problematic. Here, we describe how a combination of biochemical and genetic techniques has been utilized to identify the molecular target of MPD in P. infestans. Phytophthora infestans germinating cysts treated with MPD produced swelling symptoms typical of cell wall synthesis inhibitors, and these effects were reversible after washing with H(2)O. Uptake studies with (14)C-labelled MPD showed that this oomycete control agent acts on the cell wall and does not enter the cell. Furthermore, (14)C glucose incorporation into cellulose was perturbed in the presence of MPD which, taken together, suggests that the inhibition of cellulose synthesis is the primary effect of MPD. Laboratory mutants, insensitive to MPD, were raised by ethyl methane sulphonate (EMS) mutagenesis, and gene sequence analysis of cellulose synthase genes in these mutants revealed two point mutations in the PiCesA3 gene, known to be involved in cellulose synthesis. Both mutations in the PiCesA3 gene result in a change to the same amino acid (glycine-1105) in the protein. The transformation and expression of a mutated PiCesA3 allele was carried out in a sensitive wild-type isolate to demonstrate that the mutations in PiCesA3 were responsible for the MPD insensitivity phenotype.
DOI: 10.1111/j.1364-3703.2009.00604.x
PubMed: 20447272
PubMed Central: PMC6640402
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Mandipropamid targets the cellulose synthase-like PiCesA3 to inhibit cell wall biosynthesis in the oomycete plant pathogen, Phytophthora infestans.</title><author><name sortKey="Blum, Mathias" sort="Blum, Mathias" uniqKey="Blum M" first="Mathias" last="Blum">Mathias Blum</name><affiliation wicri:level="1"><nlm:affiliation>Syngenta Crop Protection AG, CH-4332 Stein, Switzerland.</nlm:affiliation><country xml:lang="fr">Suisse</country><wicri:regionArea>Syngenta Crop Protection AG, CH-4332 Stein</wicri:regionArea><wicri:noRegion>CH-4332 Stein</wicri:noRegion></affiliation></author><author><name sortKey="Boehler, Martine" sort="Boehler, Martine" uniqKey="Boehler M" first="Martine" last="Boehler">Martine Boehler</name></author><author><name sortKey="Randall, Eva" sort="Randall, Eva" uniqKey="Randall E" first="Eva" last="Randall">Eva Randall</name></author><author><name sortKey="Young, Vanessa" sort="Young, Vanessa" uniqKey="Young V" first="Vanessa" last="Young">Vanessa Young</name></author><author><name sortKey="Csukai, Michael" sort="Csukai, Michael" uniqKey="Csukai M" first="Michael" last="Csukai">Michael Csukai</name></author><author><name sortKey="Kraus, Sabrina" sort="Kraus, Sabrina" uniqKey="Kraus S" first="Sabrina" last="Kraus">Sabrina Kraus</name></author><author><name sortKey="Moulin, Florence" sort="Moulin, Florence" uniqKey="Moulin F" first="Florence" last="Moulin">Florence Moulin</name></author><author><name sortKey="Scalliet, Gabriel" sort="Scalliet, Gabriel" uniqKey="Scalliet G" first="Gabriel" last="Scalliet">Gabriel Scalliet</name></author><author><name sortKey="Avrova, Anna O" sort="Avrova, Anna O" uniqKey="Avrova A" first="Anna O" last="Avrova">Anna O. Avrova</name></author><author><name sortKey="Whisson, Stephen C" sort="Whisson, Stephen C" uniqKey="Whisson S" first="Stephen C" last="Whisson">Stephen C. Whisson</name></author><author><name sortKey="Fonne Pfister, Raymonde" sort="Fonne Pfister, Raymonde" uniqKey="Fonne Pfister R" first="Raymonde" last="Fonne-Pfister">Raymonde Fonne-Pfister</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2010">2010</date><idno type="RBID">pubmed:20447272</idno><idno type="pmid">20447272</idno><idno type="doi">10.1111/j.1364-3703.2009.00604.x</idno><idno type="pmc">PMC6640402</idno><idno type="wicri:Area/Main/Corpus">001912</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">001912</idno><idno type="wicri:Area/Main/Curation">001912</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">001912</idno><idno type="wicri:Area/Main/Exploration">001912</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Mandipropamid targets the cellulose synthase-like PiCesA3 to inhibit cell wall biosynthesis in the oomycete plant pathogen, Phytophthora infestans.</title><author><name sortKey="Blum, Mathias" sort="Blum, Mathias" uniqKey="Blum M" first="Mathias" last="Blum">Mathias Blum</name><affiliation wicri:level="1"><nlm:affiliation>Syngenta Crop Protection AG, CH-4332 Stein, Switzerland.</nlm:affiliation><country xml:lang="fr">Suisse</country><wicri:regionArea>Syngenta Crop Protection AG, CH-4332 Stein</wicri:regionArea><wicri:noRegion>CH-4332 Stein</wicri:noRegion></affiliation></author><author><name sortKey="Boehler, Martine" sort="Boehler, Martine" uniqKey="Boehler M" first="Martine" last="Boehler">Martine Boehler</name></author><author><name sortKey="Randall, Eva" sort="Randall, Eva" uniqKey="Randall E" first="Eva" last="Randall">Eva Randall</name></author><author><name sortKey="Young, Vanessa" sort="Young, Vanessa" uniqKey="Young V" first="Vanessa" last="Young">Vanessa Young</name></author><author><name sortKey="Csukai, Michael" sort="Csukai, Michael" uniqKey="Csukai M" first="Michael" last="Csukai">Michael Csukai</name></author><author><name sortKey="Kraus, Sabrina" sort="Kraus, Sabrina" uniqKey="Kraus S" first="Sabrina" last="Kraus">Sabrina Kraus</name></author><author><name sortKey="Moulin, Florence" sort="Moulin, Florence" uniqKey="Moulin F" first="Florence" last="Moulin">Florence Moulin</name></author><author><name sortKey="Scalliet, Gabriel" sort="Scalliet, Gabriel" uniqKey="Scalliet G" first="Gabriel" last="Scalliet">Gabriel Scalliet</name></author><author><name sortKey="Avrova, Anna O" sort="Avrova, Anna O" uniqKey="Avrova A" first="Anna O" last="Avrova">Anna O. Avrova</name></author><author><name sortKey="Whisson, Stephen C" sort="Whisson, Stephen C" uniqKey="Whisson S" first="Stephen C" last="Whisson">Stephen C. Whisson</name></author><author><name sortKey="Fonne Pfister, Raymonde" sort="Fonne Pfister, Raymonde" uniqKey="Fonne Pfister R" first="Raymonde" last="Fonne-Pfister">Raymonde Fonne-Pfister</name></author></analytic><series><title level="j">Molecular plant pathology</title><idno type="eISSN">1364-3703</idno><imprint><date when="2010" type="published">2010</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Algal Proteins (chemistry)</term><term>Algal Proteins (genetics)</term><term>Algal Proteins (metabolism)</term><term>Amides (pharmacology)</term><term>Amino Acid Sequence (MeSH)</term><term>Carboxylic Acids (pharmacology)</term><term>Cell Wall (drug effects)</term><term>Cell Wall (metabolism)</term><term>Cellulose (biosynthesis)</term><term>Crosses, Genetic (MeSH)</term><term>Ethyl Methanesulfonate (MeSH)</term><term>Gene Dosage (genetics)</term><term>Glucose (metabolism)</term><term>Glucosyltransferases (chemistry)</term><term>Glucosyltransferases (genetics)</term><term>Glucosyltransferases (metabolism)</term><term>Molecular Sequence Data (MeSH)</term><term>Mutagenesis (drug effects)</term><term>Mutation (genetics)</term><term>Phytophthora infestans (cytology)</term><term>Phytophthora infestans (drug effects)</term><term>Phytophthora infestans (enzymology)</term><term>Phytophthora infestans (genetics)</term><term>Plants (drug effects)</term><term>Plants (microbiology)</term><term>Transformation, Genetic (drug effects)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Acides carboxyliques (pharmacologie)</term><term>Amides (pharmacologie)</term><term>Cellulose (biosynthèse)</term><term>Croisements génétiques (MeSH)</term><term>Données de séquences moléculaires (MeSH)</term><term>Dosage génique (génétique)</term><term>Glucose (métabolisme)</term><term>Glucosyltransferases (composition chimique)</term><term>Glucosyltransferases (génétique)</term><term>Glucosyltransferases (métabolisme)</term><term>Mutagenèse (effets des médicaments et des substances chimiques)</term><term>Mutation (génétique)</term><term>Méthanesulfonate d'éthyle (MeSH)</term><term>Paroi cellulaire (effets des médicaments et des substances chimiques)</term><term>Paroi cellulaire (métabolisme)</term><term>Phytophthora infestans (cytologie)</term><term>Phytophthora infestans (effets des médicaments et des substances chimiques)</term><term>Phytophthora infestans (enzymologie)</term><term>Phytophthora infestans (génétique)</term><term>Plantes (effets des médicaments et des substances chimiques)</term><term>Plantes (microbiologie)</term><term>Protéines d'algue (composition chimique)</term><term>Protéines d'algue (génétique)</term><term>Protéines d'algue (métabolisme)</term><term>Séquence d'acides aminés (MeSH)</term><term>Transformation génétique (effets des médicaments et des substances chimiques)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Cellulose</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Algal Proteins</term><term>Glucosyltransferases</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Algal Proteins</term><term>Glucosyltransferases</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Algal Proteins</term><term>Glucose</term><term>Glucosyltransferases</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Amides</term><term>Carboxylic Acids</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Cellulose</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Glucosyltransferases</term><term>Protéines d'algue</term></keywords><keywords scheme="MESH" qualifier="cytologie" xml:lang="fr"><term>Phytophthora infestans</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Phytophthora infestans</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Cell Wall</term><term>Mutagenesis</term><term>Phytophthora infestans</term><term>Plants</term><term>Transformation, Genetic</term></keywords><keywords scheme="MESH" qualifier="effets des médicaments et des substances chimiques" xml:lang="fr"><term>Mutagenèse</term><term>Paroi cellulaire</term><term>Phytophthora infestans</term><term>Plantes</term><term>Transformation génétique</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Phytophthora infestans</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Phytophthora infestans</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Gene Dosage</term><term>Mutation</term><term>Phytophthora infestans</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Dosage génique</term><term>Glucosyltransferases</term><term>Mutation</term><term>Phytophthora infestans</term><term>Protéines d'algue</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Cell Wall</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Plantes</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Plants</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Glucose</term><term>Glucosyltransferases</term><term>Paroi cellulaire</term><term>Protéines d'algue</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Acides carboxyliques</term><term>Amides</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Crosses, Genetic</term><term>Ethyl Methanesulfonate</term><term>Molecular Sequence Data</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Croisements génétiques</term><term>Données de séquences moléculaires</term><term>Méthanesulfonate d'éthyle</term><term>Séquence d'acides aminés</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Oomycete plant pathogens cause a wide variety of economically and environmentally important plant diseases. Mandipropamid (MPD) is a carboxylic acid amide (CAA) effective against downy mildews, such as Plasmopara viticola on grapes and potato late blight caused by Phytophthora infestans. Historically, the identification of the mode of action of oomycete-specific control agents has been problematic. Here, we describe how a combination of biochemical and genetic techniques has been utilized to identify the molecular target of MPD in P. infestans. Phytophthora infestans germinating cysts treated with MPD produced swelling symptoms typical of cell wall synthesis inhibitors, and these effects were reversible after washing with H(2)O. Uptake studies with (14)C-labelled MPD showed that this oomycete control agent acts on the cell wall and does not enter the cell. Furthermore, (14)C glucose incorporation into cellulose was perturbed in the presence of MPD which, taken together, suggests that the inhibition of cellulose synthesis is the primary effect of MPD. Laboratory mutants, insensitive to MPD, were raised by ethyl methane sulphonate (EMS) mutagenesis, and gene sequence analysis of cellulose synthase genes in these mutants revealed two point mutations in the PiCesA3 gene, known to be involved in cellulose synthesis. Both mutations in the PiCesA3 gene result in a change to the same amino acid (glycine-1105) in the protein. The transformation and expression of a mutated PiCesA3 allele was carried out in a sensitive wild-type isolate to demonstrate that the mutations in PiCesA3 were responsible for the MPD insensitivity phenotype.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">20447272</PMID><DateCompleted><Year>2010</Year><Month>09</Month><Day>27</Day></DateCompleted><DateRevised><Year>2020</Year><Month>02</Month><Day>25</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1364-3703</ISSN><JournalIssue CitedMedium="Internet"><Volume>11</Volume><Issue>2</Issue><PubDate><Year>2010</Year><Month>Mar</Month></PubDate></JournalIssue><Title>Molecular plant pathology</Title><ISOAbbreviation>Mol Plant Pathol</ISOAbbreviation></Journal><ArticleTitle>Mandipropamid targets the cellulose synthase-like PiCesA3 to inhibit cell wall biosynthesis in the oomycete plant pathogen, Phytophthora infestans.</ArticleTitle><Pagination><MedlinePgn>227-43</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1111/j.1364-3703.2009.00604.x</ELocationID><Abstract><AbstractText>Oomycete plant pathogens cause a wide variety of economically and environmentally important plant diseases. Mandipropamid (MPD) is a carboxylic acid amide (CAA) effective against downy mildews, such as Plasmopara viticola on grapes and potato late blight caused by Phytophthora infestans. Historically, the identification of the mode of action of oomycete-specific control agents has been problematic. Here, we describe how a combination of biochemical and genetic techniques has been utilized to identify the molecular target of MPD in P. infestans. Phytophthora infestans germinating cysts treated with MPD produced swelling symptoms typical of cell wall synthesis inhibitors, and these effects were reversible after washing with H(2)O. Uptake studies with (14)C-labelled MPD showed that this oomycete control agent acts on the cell wall and does not enter the cell. Furthermore, (14)C glucose incorporation into cellulose was perturbed in the presence of MPD which, taken together, suggests that the inhibition of cellulose synthesis is the primary effect of MPD. Laboratory mutants, insensitive to MPD, were raised by ethyl methane sulphonate (EMS) mutagenesis, and gene sequence analysis of cellulose synthase genes in these mutants revealed two point mutations in the PiCesA3 gene, known to be involved in cellulose synthesis. Both mutations in the PiCesA3 gene result in a change to the same amino acid (glycine-1105) in the protein. The transformation and expression of a mutated PiCesA3 allele was carried out in a sensitive wild-type isolate to demonstrate that the mutations in PiCesA3 were responsible for the MPD insensitivity phenotype.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Blum</LastName><ForeName>Mathias</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Syngenta Crop Protection AG, CH-4332 Stein, Switzerland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Boehler</LastName><ForeName>Martine</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Randall</LastName><ForeName>Eva</ForeName><Initials>E</Initials></Author><Author ValidYN="Y"><LastName>Young</LastName><ForeName>Vanessa</ForeName><Initials>V</Initials></Author><Author ValidYN="Y"><LastName>Csukai</LastName><ForeName>Michael</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Kraus</LastName><ForeName>Sabrina</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Moulin</LastName><ForeName>Florence</ForeName><Initials>F</Initials></Author><Author ValidYN="Y"><LastName>Scalliet</LastName><ForeName>Gabriel</ForeName><Initials>G</Initials></Author><Author ValidYN="Y"><LastName>Avrova</LastName><ForeName>Anna O</ForeName><Initials>AO</Initials></Author><Author ValidYN="Y"><LastName>Whisson</LastName><ForeName>Stephen C</ForeName><Initials>SC</Initials></Author><Author ValidYN="Y"><LastName>Fonne-Pfister</LastName><ForeName>Raymonde</ForeName><Initials>R</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Mol Plant Pathol</MedlineTA><NlmUniqueID>100954969</NlmUniqueID><ISSNLinking>1364-3703</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D020418">Algal Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000577">Amides</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D002264">Carboxylic Acids</NameOfSubstance></Chemical><Chemical><RegistryNumber>11GP4ELK0U</RegistryNumber><NameOfSubstance UI="C550943">mandipropamid</NameOfSubstance></Chemical><Chemical><RegistryNumber>9004-34-6</RegistryNumber><NameOfSubstance UI="D002482">Cellulose</NameOfSubstance></Chemical><Chemical><RegistryNumber>9H154DI0UP</RegistryNumber><NameOfSubstance UI="D005020">Ethyl Methanesulfonate</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 2.4.1.-</RegistryNumber><NameOfSubstance UI="D005964">Glucosyltransferases</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 2.4.1.-</RegistryNumber><NameOfSubstance UI="C478648">cellulose synthase</NameOfSubstance></Chemical><Chemical><RegistryNumber>IY9XDZ35W2</RegistryNumber><NameOfSubstance UI="D005947">Glucose</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D020418" MajorTopicYN="N">Algal Proteins</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000577" MajorTopicYN="N">Amides</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000595" MajorTopicYN="N">Amino Acid Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002264" MajorTopicYN="N">Carboxylic Acids</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002473" MajorTopicYN="N">Cell Wall</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002482" MajorTopicYN="N">Cellulose</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="N">biosynthesis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D003433" MajorTopicYN="N">Crosses, Genetic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005020" MajorTopicYN="N">Ethyl Methanesulfonate</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018628" MajorTopicYN="N">Gene Dosage</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005947" MajorTopicYN="N">Glucose</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005964" MajorTopicYN="N">Glucosyltransferases</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D016296" MajorTopicYN="N">Mutagenesis</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009154" MajorTopicYN="N">Mutation</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D055750" MajorTopicYN="N">Phytophthora infestans</DescriptorName><QualifierName UI="Q000166" MajorTopicYN="N">cytology</QualifierName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010944" MajorTopicYN="N">Plants</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014170" MajorTopicYN="N">Transformation, Genetic</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2010</Year><Month>5</Month><Day>8</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2010</Year><Month>5</Month><Day>8</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2010</Year><Month>9</Month><Day>29</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">20447272</ArticleId><ArticleId IdType="pii">MPP604</ArticleId><ArticleId IdType="doi">10.1111/j.1364-3703.2009.00604.x</ArticleId><ArticleId IdType="pmc">PMC6640402</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Plant Physiol. 2001 Apr;125(4):2040-52</Citation><ArticleIdList><ArticleId IdType="pubmed">11299383</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 2001 Jul;126(3):981-92</Citation><ArticleIdList><ArticleId IdType="pubmed">11457949</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2001 Aug 28;98(18):10079-84</Citation><ArticleIdList><ArticleId IdType="pubmed">11517344</ArticleId></ArticleIdList></Reference><Reference><Citation>Pest Manag Sci. 2001 Nov;57(11):1081-7</Citation><ArticleIdList><ArticleId IdType="pubmed">11721527</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 2002 Feb;128(2):482-90</Citation><ArticleIdList><ArticleId IdType="pubmed">11842152</ArticleId></ArticleIdList></Reference><Reference><Citation>Phytochemistry. 2002 Apr;59(7):689-96</Citation><ArticleIdList><ArticleId IdType="pubmed">11909624</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell Physiol. 2002 Dec;43(12):1407-20</Citation><ArticleIdList><ArticleId IdType="pubmed">12514238</ArticleId></ArticleIdList></Reference><Reference><Citation>Protoplasma. 2003 Mar;220(3-4):201-7</Citation><ArticleIdList><ArticleId IdType="pubmed">12664284</ArticleId></ArticleIdList></Reference><Reference><Citation>Genetics. 2003 Jun;164(2):731-40</Citation><ArticleIdList><ArticleId IdType="pubmed">12807792</ArticleId></ArticleIdList></Reference><Reference><Citation>Fungal Genet Biol. 2003 Oct;40(1):4-14</Citation><ArticleIdList><ArticleId IdType="pubmed">12948509</ArticleId></ArticleIdList></Reference><Reference><Citation>Eur J Biochem. 2004 Sep;271(18):3635-45</Citation><ArticleIdList><ArticleId IdType="pubmed">15355340</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Microbiol. 2005 May;56(3):638-48</Citation><ArticleIdList><ArticleId IdType="pubmed">15819621</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2005 May 24;102(21):7766-71</Citation><ArticleIdList><ArticleId IdType="pubmed">15894622</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 1977 Jun;59(6):1088-97</Citation><ArticleIdList><ArticleId IdType="pubmed">16660000</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 1990 Jun;93(2):695-700</Citation><ArticleIdList><ArticleId IdType="pubmed">16667525</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Microbiol. 2006 Oct;62(2):552-65</Citation><ArticleIdList><ArticleId IdType="pubmed">16978258</ArticleId></ArticleIdList></Reference><Reference><Citation>Cell Microbiol. 2007 Jan;9(1):31-9</Citation><ArticleIdList><ArticleId IdType="pubmed">17081190</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Plant Microbe Interact. 2006 Dec;19(12):1348-58</Citation><ArticleIdList><ArticleId IdType="pubmed">17153919</ArticleId></ArticleIdList></Reference><Reference><Citation>FEMS Microbiol Lett. 2007 Sep;274(1):1-8</Citation><ArticleIdList><ArticleId IdType="pubmed">17559387</ArticleId></ArticleIdList></Reference><Reference><Citation>Nature. 2007 Nov 1;450(7166):115-8</Citation><ArticleIdList><ArticleId IdType="pubmed">17914356</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Plant Microbe Interact. 1991 Nov-Dec;4(6):602-7</Citation><ArticleIdList><ArticleId IdType="pubmed">1804404</ArticleId></ArticleIdList></Reference><Reference><Citation>Pest Manag Sci. 2008 Mar;64(3):255-61</Citation><ArticleIdList><ArticleId IdType="pubmed">18095384</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Plant Microbe Interact. 2008 Apr;21(4):433-47</Citation><ArticleIdList><ArticleId IdType="pubmed">18321189</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell. 2008 Mar;20(3):720-38</Citation><ArticleIdList><ArticleId IdType="pubmed">18349153</ArticleId></ArticleIdList></Reference><Reference><Citation>Cell Microbiol. 2008 Nov;10(11):2271-84</Citation><ArticleIdList><ArticleId IdType="pubmed">18637942</ArticleId></ArticleIdList></Reference><Reference><Citation>Trends Parasitol. 2008 Nov;24(11):502-8</Citation><ArticleIdList><ArticleId IdType="pubmed">18805734</ArticleId></ArticleIdList></Reference><Reference><Citation>J Exp Bot. 2008;59(14):3963-74</Citation><ArticleIdList><ArticleId IdType="pubmed">18832186</ArticleId></ArticleIdList></Reference><Reference><Citation>Phytopathology. 2007 May;97(5):643-9</Citation><ArticleIdList><ArticleId IdType="pubmed">18943584</ArticleId></ArticleIdList></Reference><Reference><Citation>Phytopathology. 2000 Nov;90(11):1201-8</Citation><ArticleIdList><ArticleId IdType="pubmed">18944421</ArticleId></ArticleIdList></Reference><Reference><Citation>Microbiology. 2008 Dec;154(Pt 12):3743-51</Citation><ArticleIdList><ArticleId IdType="pubmed">19047742</ArticleId></ArticleIdList></Reference><Reference><Citation>Genome Biol. 2009;10(4):R36</Citation><ArticleIdList><ArticleId IdType="pubmed">19368726</ArticleId></ArticleIdList></Reference><Reference><Citation>New Phytol. 2009;184(1):114-26</Citation><ArticleIdList><ArticleId IdType="pubmed">19645738</ArticleId></ArticleIdList></Reference><Reference><Citation>Nature. 2009 Sep 17;461(7262):393-8</Citation><ArticleIdList><ArticleId IdType="pubmed">19741609</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Plant Pathol. 2005 Mar 1;6(2):153-63</Citation><ArticleIdList><ArticleId IdType="pubmed">20565646</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 1988 Jan 29;239(4839):487-91</Citation><ArticleIdList><ArticleId IdType="pubmed">2448875</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Dis. 2008 May;92(5):675-683</Citation><ArticleIdList><ArticleId IdType="pubmed">30769584</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Dis. 2004 Sep;88(9):930-934</Citation><ArticleIdList><ArticleId IdType="pubmed">30812244</ArticleId></ArticleIdList></Reference><Reference><Citation>Annu Rev Microbiol. 1968;22:87-108</Citation><ArticleIdList><ArticleId IdType="pubmed">4879523</ArticleId></ArticleIdList></Reference><Reference><Citation>Anal Biochem. 1969 Dec;32(3):420-4</Citation><ArticleIdList><ArticleId IdType="pubmed">5361396</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant J. 1993 Jan;3(1):1-30</Citation><ArticleIdList><ArticleId IdType="pubmed">8401598</ArticleId></ArticleIdList></Reference><Reference><Citation>Genetics. 1996 Nov;144(3):1005-13</Citation><ArticleIdList><ArticleId IdType="pubmed">8913745</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 1998 Mar;116(3):1043-51</Citation><ArticleIdList><ArticleId IdType="pubmed">9501137</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>Suisse</li></country></list><tree><noCountry><name sortKey="Avrova, Anna O" sort="Avrova, Anna O" uniqKey="Avrova A" first="Anna O" last="Avrova">Anna O. Avrova</name><name sortKey="Boehler, Martine" sort="Boehler, Martine" uniqKey="Boehler M" first="Martine" last="Boehler">Martine Boehler</name><name sortKey="Csukai, Michael" sort="Csukai, Michael" uniqKey="Csukai M" first="Michael" last="Csukai">Michael Csukai</name><name sortKey="Fonne Pfister, Raymonde" sort="Fonne Pfister, Raymonde" uniqKey="Fonne Pfister R" first="Raymonde" last="Fonne-Pfister">Raymonde Fonne-Pfister</name><name sortKey="Kraus, Sabrina" sort="Kraus, Sabrina" uniqKey="Kraus S" first="Sabrina" last="Kraus">Sabrina Kraus</name><name sortKey="Moulin, Florence" sort="Moulin, Florence" uniqKey="Moulin F" first="Florence" last="Moulin">Florence Moulin</name><name sortKey="Randall, Eva" sort="Randall, Eva" uniqKey="Randall E" first="Eva" last="Randall">Eva Randall</name><name sortKey="Scalliet, Gabriel" sort="Scalliet, Gabriel" uniqKey="Scalliet G" first="Gabriel" last="Scalliet">Gabriel Scalliet</name><name sortKey="Whisson, Stephen C" sort="Whisson, Stephen C" uniqKey="Whisson S" first="Stephen C" last="Whisson">Stephen C. Whisson</name><name sortKey="Young, Vanessa" sort="Young, Vanessa" uniqKey="Young V" first="Vanessa" last="Young">Vanessa Young</name></noCountry><country name="Suisse"><noRegion><name sortKey="Blum, Mathias" sort="Blum, Mathias" uniqKey="Blum M" first="Mathias" last="Blum">Mathias Blum</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/PhytophthoraV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001885 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 001885 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= PhytophthoraV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:20447272
|texte= Mandipropamid targets the cellulose synthase-like PiCesA3 to inhibit cell wall biosynthesis in the oomycete plant pathogen, Phytophthora infestans.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:20447272" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a PhytophthoraV1
| This area was generated with Dilib version V0.6.38. |  |